| 释义 |
shRNA
基本例句
短发夹RNA¹⁰⁰
ObjectiveTo establish spinal muscular atrophy cell model by blocking the expression of SMN1 gene withshRNA.目的：应用RNA干扰沉默SMN1基因的表达建立脊髓性肌萎缩症SMA的细胞模型。
TheshRNAexpression vector can effectively inhibit the expression of Beclin 1 in the HeLa cells, and promote the growth of HeLa cells.成功构建了针对自噬基因Beclin 1的shRNA表达载体,通过转染HeLa细胞,可有效抑制细胞中Beclin 1的表达,并促进细胞生长,抑制凋亡。
ResultsshRNAof PCNA could significantly inhibit the growth of HeLa cells and arrest HeLa cells in G0/G1-S phase. Also the expression of PCNA protein was inhibited.结果PCNAshRNA能显著抑制人HeLa细胞的生长,阻滞细胞于G0/G1期,PCNA的蛋白表达同时受到抑制。
Objctive:It is surveyed whether the recombinant adenovirus vector pAdeno-X-siFas 1+siFas 2 expressing two Fas-targeted short hairpin RNAs could supress the overexpression of Fas induced by LPS/D-GalN in BALB/c mouse liver.目的:观察串联表达Fas-shRNA的腺病毒载体pAdeno-X-siFas 1+siFas 2对脂多糖/D-氨基半乳糖诱导BALB/c鼠肝细胞Fas过表达的影响。
METHODS: TheshRNAspecific to PTI-1 was synthesized and inserted into pEGFP/U6 by gene recombination technology to construct the expression vector, pEGFP/U6-mPs, which was then transfected into the cultured PC-3 cells.方法:采用基因克隆技术,将合成的短发卡样特异性PTI1RNA干扰寡核苷酸序列插入真核表达载体pEGFP/U6,构建PTI1shRNA的真核表达载体,体外转染人前列腺癌PC3细胞,48h后观察细胞生物学变化;
Methods Survivin-targetingshRNAeukaryotic expression vector pRNAT-U6. 1/neo-survivin was constructed and identified. Human bladder cancer cell T24 was transfect-ed with pRNAT-U6. 1/neo-survivin and then stable monoclonal cells were selected.方法构建Survivin基因shRNA真核表达载体pRNAT-U6.;1/neo-survivin;转染人膀胱癌细胞T24并获得稳定的单克隆细胞。 |