释义 |
restriction endonuclease短语⁹⁶⁶⁷⁷⁺⁶ 基本英英近义反义例句例句 限制性核酸内切酶,限制性内切酶
Noun: any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria where they cripple viral invaders; used in recombinant DNA technology近义词 restriction nuclease限制性核酸酶…restriction enzyme限制性内切酶,限制酶… It enables a specific gene to be located on a particular restriction enzyme fragment.它就能使专一的基因被定位于特定的限制性内切酶切成的片段上。 Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation.根据揭示多态性的限制性内切酶的数量可将产生的突变大多归为点突变。 Finally, we could evaluate plasmids cDNA extracted with mono- restriction endonuclease enzyme and the agar gel electrophoresis. 最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。 cnki The recombinant plasmid PET- 11D- ospC was identified with restriction endonuclease analysis and sequencing. 采用酶切分析及序列测定等方法鉴定重组质粒的正确性。 www.bmsw.net.cn The recombined clones were identified by agarose gel electrophoresis after restriction endonuclease digesting. 重组子通过特异限制性内切酶切割,琼脂糖电泳予以鉴定。 cnki After a restriction endonuclease cleavage, two expected smaller fragments were observed. 经限制性内切酶反应,获得两个预期大小的片段。 cnki Finally, the cloned B antigen gene was identified by restriction endonuclease analysis, DIG probe hybridization and DNA sequencing. 通过内切酶分析、地高辛 DIG—探针原位杂交、 DNA序列测定,确认克隆的 B抗原基因正确。 cnki In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis. 本研究利用多种组合的酶切分析,绘制了较为详细的酶切图谱。 cnki Methods: DNA sequencing and restriction endonuclease reaction was used. 方法:采用 DNA测序及限制性酶切反应方法。 cnki Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls; 并对发现突变的基因进行分析。方法应用PCR扩增、 DNA序列测定等技术分析所有 AR基因外显子及其邻近 DNA序列片段; doi Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification. 结果经酶切鉴定及基因测序证实重组三突变型穿梭质粒构建成功。 journal.cmu.edu.cn Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful. 结果酶切及测序显示对 OSCP基因有义突变部分的纠正获得成功。 chemyq |