释义 |
refolding 英ri:'fəuldiŋ美ri:'fəuldiŋ COCA¹⁰⁵⁴⁴⁷BNC¹⁰⁴⁵⁸⁴⁺⁶ 基本例句 重褶皱作用¹⁰⁰原型refold的现在分词 In this article, we describe the basic mechanism of cloning, refolding, expressing of PLC, and the purification of recombinant PLC. 该文叙述了磷脂酶C的克隆和表达、重构的工程菌株及重组磷脂酶C的纯化制备。 chemyq Protein refolding by dilution is the basis of research on other method of renaturation. 稀释复性法是研究其他方法的基础。 cnki Recently the refolding studies have been concentrated on alkaline phosphatase from E. 对小牛肠碱性磷酸酶再折叠的机制及其影响因素进行了探讨。 dictall The unfolding and refolding of bovine serum albumins and the existence of intermediates were studied by fluorescence phase diagram. 荧光相图法研究牛血清白蛋白变性和复性过程,捕捉部分折叠中间态。 cnki The conclusion of this study will be strongly helpful to understand the mechanism of the protein refolding. 本研究结果为探索蛋白质折叠机理提供了有益的帮助。 fabiao The experimental results of urea gradient electrophoresis show that there are two different refolding processes. 尿素浓度梯度电泳实验结果显示,存在两条不同的折叠途径。 cnki The formation of aggregate occurred during unfolding and refolding. 变性与复性过程中可能伴有聚集体生成; cjbmb.bjmu.edu.cn The research into protein refolding techniques is of essential importance to the down stream processing of genetically engineered proteins. 蛋白质复性技术的研究对于基因工程重组蛋白质的生产具有重要意义。 dictall The results showed that the refolding efficiency of proteins denatured by GuHCl is the highest, and SDS is the lowest in the three denaturing agents. 盐酸胍变性的蛋白质复性后的质量回收率和活性回收率均高于尿素, SDS变性的蛋白质构象难以恢复,复性效果最差。 cnki Calcium and zinc ions were required both in refolding and enzymatic activity, but high concentration of zinc inhibited the refolding and activity. 该蛋白的重折叠复性和酶活性需要钙锌离子,但高浓度的锌离子则抑制其重折叠复性和活性。 cnki Conclusion The refolding of sHLA- G1- peptide complex can be successfully generated with Prokaryotic expressing and Refolding in vitro. 结论通过原核表达、体外折叠能够获得大量可溶性HLA- G1-肽复合物。 cnki Conclusion The parameters of expression, purification and refolding of sCR1- SCR15-18 protein were optimized, which may pave a way for further studies. 结论优化了 sCR1- SCR15-18蛋白的表达、纯化和复性的参数,所获结果为进一步动物体内保护实验研究奠定了基础。 cnki In addition, in the presence of the artificialchaperones, lysozyme refolding could be realized at high yield and folding rate at alow GdmCl concentration. 此外,若有人工伴侣的存在,在较低浓度的盐酸胍条件下就可实现快速、高收率的蛋白质复性。 cnki In this chapter solute retention mechanism in LC, protein refolding mechanism and new developments in protein refolding kinetics were reviewed. It contains61 references. 本章对色谱中的保留机理及理论、蛋白质液相色谱复性机理及蛋白折叠动力学理论模型研究进展进行了综述,共包括文献61篇。 cnki In this thesis, unfolding and refolding of human group IB and X phospholipase A2 have been studied. 本论文以人的 IB和 X型磷脂酶 A2 为研究对象,研究了蛋白质的去折叠和重折叠反应。 cnki Investigations on protein refolding by size-exclusion chromatographySEC was carried out by using lysozyme as a model system. 以溶菌酶为模拟体系对体积排阻色谱法进行蛋白质折叠过程实验研究。 dictall Kinetic analysis showed that, in the presence of the artificial system, the aggregate formation was a third order reaction, the same as that of the refolding by direct dilution. 动力学分析表明,人工伴侣存在下聚集体的生成为三级反应,与直接稀释复性相同; cnki Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory. 在已研究过的蛋白质中,大多数蛋白质都是沿着多途径而非单一、特定的途径进行氧化重折叠,这与折叠能量景观学说是一致的。 cnki Recently, with the development of studying in mechanism of protein refolding, some new strategies were reported to solve this problem. 近年来,随着对包涵体体外复性机制的深入研究,从包涵体中复性重组蛋白质已经有了很多的策略和方法。 chemyq The denatured recombinant S receptor- binding region protein could not induce neutralizing antibodies, whereas refolding recombinant S-RBD could elicit neutralizing antibodies. 实验表明, S蛋白受体结合区无线性中和表位,中和抗体的产生是由构象表位诱导的。 virol The kinetic model was in good agreement with the experimental results. The mechanism of CAB refolding assisted by amides were analogous to that of GdmCl. 利用三级反应动力学方程拟和酰胺化合物辅助 CAB复性的动力学过程。结果表明,模型计算值与实验数据能较好吻合。 cnki The recent research progress of recombinant protein refolding was reviewed. 本文对近年来重组蛋白复性技术的研究进行了评述。 cnki Then according to experiment design, the effects of rChIL-18 protein refolding yield at different densities were investigated by the systems of artificial chaperone at different densities. 然后按照试验设计,考察不同浓度组成的人工分子伴侣体系对不同浓度鸡 IL-18重组蛋白复性率的影响。 dictall These inclusion bodies should be washed with denaturation buffer firstly, then followed by denaturation and refolding in proper buffer to harvest the active proteins. 包涵体中富含表达的重组蛋白质,它们经分离、洗涤、变性溶解以及复性等过程才能得到具有生物活性的蛋白质。 chemyq This work studied refolding kinetics of denatured lysozyme by fed-batch operation with dilution additives. 本文研究了添加剂与流加操作辅助变性溶菌酶复性动力学。 cnki |