释义 |
rAAV 基本例句 腺相关病毒¹⁰⁰ In the present study, recombinant adeno-associated virus was employed as a gene delivery vector to study glucose regulated insulin gene therapy.在本研究之中将利用重组腺相关病毒作为基因载体,以研究在体外及体内试验中受葡萄糖调控的胰岛素生产情形。 EPC was efficiently infected byrAAVencoding and was expressed by EPCs,which establishes a basis for therapeutic angiogenesis by transgenic EPCs transplantation.编码sp-haFGF的重组腺相关病毒能够有效地感染EPC,并在EPC中表达haFGF,为进一步研究转基因的内皮祖细胞移植促血管新生奠定基础。 AIM: To observe the expression of recombinated adeno-associated virus 1/2-lacZ vector gene in umbilical mesenchymal stem cells cultured in vitro.目的:观察由重组腺相关病毒1/2载体携带的LacZ报告基因在体外培养的脐血间质干细胞中的表达情况。 Renalembryo 293 cell was co-transfected with therAAVvector of plasmid pSSHG/NT4-GFP-Ant ,packaging plasmid pAAV/ Ad and helper adeno virus pasmidpFG140.The recombinant AAV viral stock was titrated by dot blot.用腺病毒辅助质粒Pfg140、包装质粒pAAV/Ad及已构建的重组腺伴随病毒载体质粒,三质粒磷酸钙共沉淀法转染80%融合的293细胞系,包装rAAV。 Objective:To deliver the gene that encodes the mature peptide of secondary lymphoid tissue chemokine into mouse bone marrow derived dendritic cell withrAAV, and to detect its anti-tumor effect.目的:应用次级淋巴组织趋化因子成熟肽基因/重组腺相关病毒转染树突状细胞,探讨一种基因修饰DC的新方法及其抗肿瘤的生物学活性。GFP expression in co-transfected AAV-293 cells was observed and the titer ofrAAVis 106particles/ml measured by that cells expressing green fluorescence were counted and compared with the total number of cells.. 2⎤ |