| 释义 |
overlapping PCR
基本例句
重叠PCR
The H chain and L chain gene of anti_HBsAg fab were connected to construct single_chain fab gene using overlap PCR techniques.采用重叠PCR技术 ,以抗乙肝表面抗原 IgG铰链区基因为Linker将Fab抗体基因的重链和轻链连接起来 ,构成单链Fab基因。
By designing four primers for anoverlapping PCR, we created a fusion gene Ddl cat encoding human TNF receptor I death domain and chloramphenicol acetyltransferase.通过设计4个引物进行重叠overlappingPCR，由此克隆了人肿瘤坏死因子受体I死亡域deathdomain与氯霉素乙酰转移酶CAT的融合蛋白基因DdLcat。
Keywords Immunotoxin;Overlapping PCR;Prokaryotic expression;免疫毒素;链重叠PCR;原核表达;
Keywords Lagurus lagurus;zona pellucida 3;overlap PCR;codon optimization;prokaryotic expression;草原兔尾鼠;卵透明带3;重叠PCR;密码子优化;原核表达; |