| 释义 |
Negative serum
基本例句
阴性血清
Treat the MOLT-4 cells with anti-HCV antibody and HCV RNA positive and negative serum, respectively.用抗HCV抗体和HCVRNA阳性及阴性血清感染MOLT-4细胞，制备细胞片进行免疫酶染色。
Methods Compare the effect of antisperm 60KD?78KD antibody positive serum and antisperm antibody negative serum on the sperm in vitro.方法使用抗60KD和抗78KD阳性血清及抗精子抗体阴性血清体外处理精子,比较它们对精子的凝集、细胞毒、穿透高度和顶体反应的影响。Negative serum, positive serum , rabbit-duck serum , HRP enzyme-labeled rabbit-duck IgG were also produced. 制备了阳性血清、阴性血清、二抗阳性血清，提纯二抗并标记HRP酶，获得合格的酶标二抗。 建立了间接ELISA检测方法：抗原稀释30倍，酶标二抗稀释50倍，血清稀释50倍，临界值为0.;5。Objective To obtain the antigenic epitopes which can affinitive bind to theHCV polyclonal antibodies by using a phage 12-mer peptide libraryand to test the efficiency of converse biopannning by using negative serum.目的筛选噬菌体展示随机12 肽库中可以与HCV 抗体阳性的血清特异性结合的序列,在HCV 表达的蛋白分子上找到这个抗原表位, 分析其序列、结构特点与功能的关系。 同时检验用阴性血清逆向筛选是否能提高筛选效率。
Methods:Applying TP-ELISA,TRUST and colloidal gold test to test 102 positive sera,220 negative sera and 5 indeterminable sera were tested by TPPA,respectively.方法:分别用TP-ELISA、TRUST、金标法对102份TPPA确证阳性血清;5份TPPA可疑血清;220份TPPA确证阴性血清进行实验;比较3种梅毒初筛方法的敏感度和特异度。结果:TP-ELISA敏感度98.;04%;特异度100%; |