释义 |
Lipofectine 基本例句 脂质体¹⁰⁰ The transfection reagent lipofectin and the mismatch control oligonucleotide did not significantly affect the current.而核酸转染剂阳离子脂质体和非配对性的寡核苷酸对电流没有显著性影响。 Objective To explore the feasibility of gene modification of the bone marrow stromal cells with lipofectin transfection.目的研究脂质体转染法对骨髓基质细胞进行基因修饰的可行性。 METHODS: The Hela cells were cultured and divided into 6 groups: Control group, Sense oligodeoxynucleotides group, Lipofectin group, AS-ODNs group including three sub-groups.方法:Hela细胞分6组培养:对照组、正义寡核苷酸组、脂质体组及反义寡核苷酸组设3个亚组。 Sense recombinant eukayotic plasmid and pIRES2-EGFP vacant vector were transfected into hEF respectively with Lipofectin reagent, and were named as hEF-hTERT and hEF-EGFP.应用脂质体转染法将pIRES2增强性绿色荧光蛋白hTERT正义重组质粒及pIRES2EGFP空载质粒分别导入hEF中,相应称为hEFhTERT和hEFEGFP。 |