释义 |
lipofectamine 基本例句 脂质体¹⁰⁰ And then, CAV2-CGS gene was transfected into MDCK cell with the participation of Lipofectamine?CAV2-CGS基因在脂质体Lipofectamine~2000介导下转染MDCK细胞系,8d后重组病毒在MDCK细胞系上实现了病毒包装。 Methods pBP-wt-PTEN recombinant plasmids were transfected into ZR-75-1 cells by Lipofectamine.方法利用脂质体介导法将重组质粒pBP-wt-PTEN导入人乳腺癌细胞ZR-75-1中,嘌呤霉素筛选稳定表达细胞株并扩增培养; The recombinant plasmid was packaged and amplified after being transfected in HEK293 cells through Lipofectamine.利用脂质体介导重组腺病毒载体转染HEK293细胞,包装出完整的腺病毒; COS7 cells were transfected with pcDNA3.1/His C-Cystatin by Lipofectamine and postive clones were identified by SDS-PAGE.脂质体介导的基因转染法将重组质粒转染COS7细胞; SDS-PAGE分析表达产物; The pEGFPTTV2 was transfected into Cos7 cell line using LipoFectamine 2000, and the transcription products of TTV ORF2 gene in the transfected cells were confirmed by RT-PCR.用脂质体介导法将pEGFPTTV2质粒DNA转染Cos7细胞,通过RT-PCR分析,证实细胞中存在ORF2基因的转录产物。 |