| 释义 |
iptgBNC¹⁴⁴⁶⁶⁴⁺³
基本例句
=isopropy-β-D-thiogalactoside 异丙基-β-D-硫代半乳糖苷
After the positive plasmid was transformed into the host cell BL21 DE3 , the target gene was successfully expressed in the form of inclusion bodies when induced with IPTG.
阳性质粒转化 BL21 DE3宿主菌， 经 IPTG诱导获得表达， 重组蛋白以包涵体的形式存在。 cnki
Have optimized the expression condition, the recombinant protein could express in high efficiency at different IPTG concentration after5 hours induction.
对表达条件优化结果表明，在诱导5小时，用不同 IPTG浓度诱导，表达产物均呈高效可溶性表达。 fabiao
Induced by IPTG, was inclusion body protein.
通过 IPTG诱导，获得包涵体表达蛋白。 fabiao
The suitable time to add IPTG was important for bacteria growth.
IPTG加入的时机对菌体的生长和耐受力的有效诱导是重要的。 cnki
The change of cultivating temperature, concentrations of IPTG, different expression time had no remarkable effects on the insoluble inclusion body.
同时，研究显示：温度、 IPTG浓度和表达时相的改变对蛋白包涵体可溶性的表达无显著的影响。 fabiao
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、 IPTG浓度等进行了优化。 fabiao
The induction temperature, induction time, and IPTG concentration were also optimized by a series of experiments. Further purification modes of this protein were also explored.
同时还进行梯度实验分别对诱导的温度、时间和 IPTG诱导时菌体浓度进行优化， 并对蛋白的纯化方案进行摸索。 actacams
Transformants were induced by IPTG, and then expressed. By DTT reduction, the soluble19 peptide was obtained from chitin affinity chromatography.
表达的融合蛋白经几丁质亲和层析、二硫苏糖醇 DTT的柱内还原，直接获得可溶性19肽。 cnki
After induced by IPTG, the recombinant SAG4 protein existed in an inclusion body form.
重组菌经 IPTG诱导后，以包涵体的形式稳定表达 SAG4。 jsczz
After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。 fabiao
And then, the antibody induction expression by IPTG was conducted. The localization of expression products was detected by sandwich ELISA.
转化大肠杆菌后诱导其表达，通过 ELISA检测产物的表达和定位。 cnki
The different conditions, such as culture media, induced time and dosage of IPTG, were used to improve the target protein expression level.
为了提高目标蛋白的表达水平，进行了条件优化，诸如培养基、诱导时间和诱导剂量。 fabiao
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
IPTG诱导该基因的表达，亲和层析和原位裂解法纯化表达产物。 cnki
The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 DE3, then after, IPTG was added to induce expression.
将构建好的ABP1及 TIR1原核表达载体，转化表达宿主菌 BL21 DE3，加入诱导物 IPTG进行诱导表达。 fabiao
The Escherichia coli containing recombinant vector expressed fusion protein of36 KD after induction by IPTG.
含重组质粒的大肠杆菌在 IPTG诱导下表达了特异性的融合蛋白，其分子量为36 kD； cnki
The fused gene of RIP- GST was induced with IPTG, and the expressed production was used as antigen for immunity to rabbit.
还对融合基因 RIP- GST进行诱导表达，以表达产物作为抗原免疫兔子。 fabiao
IPTG inducing expression with host bacterium of BL21 is done.
以大肠杆菌BL21为宿主菌进行 IPTG诱导表达。 cnki |