释义 |
EGFP 基本例句 绿色荧光蛋白¹⁰⁰ EGFPand TK were successfully fused and cloned into pBABE-puro-lox to construct a new vector called pBGTKlox.成功拼接EGFP-TK融合基因并克隆入pBABE-puro-lox,构建成新栽体pBGTKlox。 The percentage of lung metastases in hEndo group,EGFPgroup and PBS group was 0.0%, 50.0% and 66.7% respectively.HENDO组、EGFP组、PBS组肺转移率分别为0·0%、50·0%、66·7%; ALR gene was inserted into pEGFP-Cl plasmid to construct ALR andEGFPco-expression vector pEGFP/ALR.ALR和EGFP融合基因表达载体,pEGFP/ALR。 Both genes were cloned into GFP expression vector pIRES2EGFPand transfected into HeLa cells.将上述基因克隆入绿色荧光蛋白表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构。 Results1. After selection by culturing with G418, clones of transgenic hMSCs were obtained and showed brightEGFPexpression.感染后的hMSCs经G418筛选后逐渐形成克隆,克隆中的hMSCs均表达EGFP,在倒置荧光显微镜下可见明亮的绿色荧光。 ALR gene was also inserted to bicistronic plasmid p! RES2-EGFPto produce ALR,EGFPgene bicistronic eukaryotic expression vector .ALR和EGFP基因双顺反子表达载体,pIRES-EGFP/ALR。 |